ENZYMES Product Basket 1 Pos no Product bags Article name pack pack size, amount size, units 25 20 30 30 20 25 10 15 15 15 15 35 15 15 15 5 15 25 5 15 35 40 10000 2000 1000 10000 4000 10000 4000 1000 1000 4000 1000 500 2000 2000 2000 1000 2000 500 500 5000 3000 5000 Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units Units 10966034 Life Tech. D8045 Sigma-Aldrich 11304011 Life Tech. 10342020 Life Tech. 100 35 25 100 300 500 100 500 rxn. U rxn. Units 18010017 M0210S M0203S M0274S 1,088E+10 P2083 P1085 5 20 15 5 5 5 10 250 200 150 300 1 000 1 000 1 000 Units Units Units Units Units Units Units 30 30 160 40 25 25 30 90 30 550 50 15 2 500 100 10 000 2 500 1 000 10 000 3 000 20 000 2 000 1 500 70 100 Units Units Units Units Units Units Units Units Units Units Kunitz units mg reference reference cat no supplier R0136S R0144S R0176S R0101S R0195S R0104S R3142S R0198S R0193S R0111S R0131S R0189S R0156S R0157S R0138S R3122S R0141S R0133S R0182S R3132L R0145S R0146S NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB NEB volumes 1.A Restriction endonucleases Restriction endonucleases BamH I Bgl II Dpn I EcoR I EcoR V Hind III Kpn I (high fidelity) Mlu I Nco I Nde I Nhe I Not I Sac I Sac II Sal I Sca I (HF) Sma I Spe I Sph I Ssp I (high fidelity) Xba I Xho I 1.B DNA polymerases for PCR Hot start polymerases for PCR Platinum Taq DNA polymerase High fidelity polymerases for P AccuTaq LA DNA polymerase Hot start high fidelity polymeraPlatinum Taq DNA polymerase High Fidelity Other polymerases for PCR Taq DNA polymerase 1.C DNA/RNA polymerases DNA polymerases RNA polymerases DNA polymerase I DNA polymerase I, large (Klenow) fragment T4 DNA polymerase T7 DNA polymerase T7 RNA polymerase T3 RNA polymerase SP6 RNA polymerase Life Tech. NEB NEB NEB Roche Promega Promega 1.D Enzymes for protein/DNA purification/modification Transferases Terminal transferase Topoisomerases/recombinase Topoisomerase I (E. coli) Reverse transcriptases Superscript II reverse transcriptase NA kinases Polynucleotide kinase (E. coli) NA phosphatases Calf intestinal phosphatase (CIP) Endonucleases S1 nuclease Exonucleases Exo I nuclease Ligases T4 DNA ligase T4 RNA ligase DNAses DNAse (RNAse-free) (50 rxn.) RNAses RNAse A from bovine pancreas Proteinase Proteinase K (5 ml) Other enzymes for protein/DNA purification/modification M0315S NEB M0301S NEB 18064014 Life Tech. M0201L NEB M0290S NEB M5761 Promega M0293S NEB M0202S NEB M0351S NEB 79254 Qiagen R6513-10MG Sigma-Aldric 25530049 Life Tech. 1.E Enzymes for Glycobiology Carbohydrate-active enzymeEndoglycosidase H (500 ul) Lysozyme from chicken egg white PNGase F 11643053001 Roche 1 3 L6876 Sigma-Aldrich 35 5 Units g P0705S NEB 25 15 000 Units 1.F Master mixes for PCR and qPCR SYBR Green master mixes Power SYBR Green PCR master mix (5 ml) Master mixes without SYBR Gr TaqMan Fast Universal PCR master mix (2X) 4367659 4352042 Life Tech. Life Tech. 120 135 reference reference volumes cat no supplier 100 bp DNA ladder 1 kb DNA ladder RNA markers NativeMark Unstained Protein Standard SeeBlue Plus2 (stained protein standard) 15628019 10787018 G3191 LC0725 LC5925 Life Tech. Life Tech. Promega Life Tech. Life Tech. dNTP set (individual dNTPs) (4 x 25 umol) dNTP mix, 10 mM each (0.5 ml) rNTP set (100 mM each (400 ul)) 10297018 Life Tech. D7295 Sigma-Aldrich E6000 Promega 200 250 rxn. rxn. Product Basket 2 BIOPOLYMERS Pos Product bags no Article name pack pack size, amount size, units 30 25 10 10 100 50 250 50 250 500 ug ug ug ul ul 40 30 5 100 5 10 umol umol umol 25 25 10 25 5 nmol nmol nmol ug nmol 1500 150 50 50 50 25 10 40 nmol ug nmol nmol 2.A Molecular weight standards DNA MW standards RNA MW standards Protein MW standards 2.B dNTP, NTP, nucleosides dNTPs NTPs 2.C Primers (standard) PCR primers and cloning oligosPrimer (tube) 20 b, 25 nmol, desalted 10629186 Life Tech. 100000 Primer (96- or 384-well plate) Primer (96- or 384-well plate), 20 primers, eac 10629152 Life Tech. 10000 Long oligonucleotides (30-120 Unmodified DNA oligo, 35 b, 10 nmol, desalteN001-001 (15ordika/Eurog 3500 T7 promoter primer, 2 ug, desalted 18418012 Life Tech. Specific sequence primers 500 1000 Random primers Primer (random hexamer), 5 nmol N8080127 Life Tech. 2.D Primers (custom), RNA oligonucleotides, synthetic genes Modified primers qPCR probes Primer 20 b (5'-Alexa-488), 50 nmol, HPLC 10623064 Life Tech. Primer (oligo(dT)12-18, 5'-phosphorylated) 25 18418012 Life Tech. qPCR probes (fluorescent end-labeled), 10 nm 450007 Life Tech. qPCR probes (double-labeled: 5'-FAM + 3'-BHQB-DD601-004 ordika/Eurog RNA oligonucleotides Synthetic genes (>120 b) 2.E DNA (genomic); RNA (total, siRNA, other) eukaryotic DNA viral DNA total RNA siRNA Other RNA Salmon sperm DNA 15632011 Life Tech. 40 5x1 ml FlexiTube siRNA 1027415 Qiagen 90 4x1 nmol 30 1 10 198 ug clones 2.F Clones and gene libraries (cDNA, PAC, BAC) cDNA clones Human cDNA ORF clones RC215923 Nordika/Orig Human Entry Collection GPCR (glycerol stock) OHS4838 Thermo Fishe 2.G Vectors Cloning vectors pBR322 Expression vectors pCDNA3.1 Reporter expression vectors Vectors for cell-free expression Reporter expression vectors Vectors for cell-free expression D1511 V79020 Promega Life Tech. 5 1 10 20 ug ug P-MANIV O-CPE Megazyme Megazyme 1 1 3 30 g mg 1 10 1 100 g ug 2.H Polysaccharides Structural polysaccharides Oligosaccharides Mannan Cellopentaose 2.I Peptides and recombinant proteins Peptides Recombinant proteins di-peptide (e.g. Ala-Ala or equivalent) A9502-1G Sigma-Aldrich Recombinant protein (E.g. Histone H10 or equ M2501S NEB 4. MOLECULAR BIOLOGY CHEMICALS pack pack size, amount size, units 25 25 25 500 500 400 ml ml ml Sigma-Aldrich Life Tech. Life Tech. Bio-Rad Life Tech. Life Tech. 80 100 100 45 125 50 25 500 500 5 10 250 ml ml ml L ml ul R90115 Life Tech. 20 25 ml P3296 Sigma-Aldrich 5 1 ml 28324-P Thermo Fisher 50 60 ml Tween-20 P5927 Sigma-Aldrich 15 100 ml Triton X-100 T8787 Sigma-Aldrich 30 100 ml 16500500 S33102 Life Tech. Life Tech. 40 125 500 400 g ul 10777019 15581044 19086 402824 12041677001 10977035 239901 Life Tech. Life Tech. Qiagen Life Tech. Roche Life Tech. Qiagen 180 100 100 25 10 300 25 5 000 1 250 25 100 500 3 U L ml ml ml ml ml 170-6435 Bio-Rad 10 1 L Pos ref ref cat no supplier 40% Acrylamide/Bisacrylamide Solution 2% Bisacrylamide Solution UltraPure 10% SDS solution 161-0148 161-0142 15553027 Bio-Rad Bio-Rad Life Tech. TEMED MOPS (20X) MES (20X) TRIS-Glycine-SDS (10X) LDS sample buffer (4X) Reducing agent (10X) T9281 NP0001 NP0002 161-0772 NP0007 NP0004 Product bags (Mol. Biol. Grade only!) volumes Article name no 4.A PAGE reagents Acrylamide, bisacrylamide Solutions Running buffers Sample buffers and reagents Transfer buffers 4.B Affinity purification Affinity resins for IMAC Protein A/G resins Other affinity resins Pre-packed columns for affinity purification Ni-NTA agarose Protein G sepharose fast flow 4.C Detergents for protein purification NP-40 (10%) Chemicals for NA synthesis, purification, modification and 4.D determination Agarose NA stain UltraPure agarose SYBR Safe (10,000X) RNAse inhibitors and surface decontaminants Buffers for NA analysis RNAseOUT RNAse inhibitor (recombinant) TBE (10X) EB Running buffer (10X) CDP-Star, ready-to-use Water, DNAse/RNAse free GelPilot DNA Loading dye (5X) NA blot detection reagents Purified/treated water Loading dyes Chemicals for protein synthesis, purification, modification and 4.E determination Blocking reagents and buffers for WB TRIS buffer saline (TBS) 10X Protease inhibitors BSA Fraction V cOmplete protease inhibitor cocktail tablets A7906-100G 4693116001 Sigma-Aldrich Roche 5 35 100 20 g each Protein inhibitors Expression inducers/screening reagents PhosSTOP phosphatase inhibitor cocktail tablets X-Gal 4906837001 V3941 5 10 20 100 each mg 5 25 g 5 50 mg Cross-linking reagents EZ-link NHS-LC-Biotin 21336 Roche Promega Saveen Werner Thermo Fisher Reducing agents DTT, aqueous solution, 1M (10 x 0.5 ml) 646563 Sigma-Aldrich 15 5 mmol ProLong Gold (or equivalent) Dextran, tetramethylrhodamine AccuCheck Counting Beads Hoechst 33342 P36931 D1818 PCB100 H3570 Life Tech. Life Tech. Life Tech. Life Tech. 60 20 20 10 10 25 10 10 ml mg ml ml IPTG I1000-25 Chemicals for flow cytometry and 4.F labeling products Antifade reagents Dextrans Fluorescent beads FC cell labels Product Basket 5 ACCESSORIES / DISPOSABLES Pos no Product bags Article name ref ref cat no supplier NP0301BOX Life Tech. volume pack each /year Size 5.A Pre-cast PAGE gels Constant percentage gels NuPAGE gels, 10-well (10%, 1.0 mm) 75 10 each NuPAGE gels, 10-well (10%, 1.5 mm) NuPAGE gels, 12-well (12%, 1.0 mm) NuPAGE gels, Tris-Acetate, 12-well (7%, 1.0 mm) Gradient gels Native gels High-throughtput gels NuPage gels, 10-well (4-12%, 1.0 mm) NuPage gels, 10-well (4-12%, 1.5 mm) NuPage gels, 12-well (4-12%, 1.0 mm) NuPage gels, 15-well (4-12%, 1.0 mm) NuPage gels, 17-well (4-12%, 1.0 mm) Novex Midi gels, 26-well (4-12%) NuPage Tris-Acetate gels, 12-well (3-8%, 1.0 mm) NativePAGE gels, 10-well (4-16%, 1.0 mm) E-PAGE 6 gels (6%) NP0315BOX NP0342BOX Life Tech. Life Tech. 100 65 10 each 10 each EA03552BOX NP0321BOX NP0335BOX NP0322BOX NP0323BOX NP0329BOX WG1403BOX Life Tech. Life Tech. Life Tech. Life Tech. Life Tech. Life Tech. Life Tech. 10 370 290 305 150 45 60 10 each 10 each 10 each 10 each 10 each 10 each 10 each EA03752BOX BN1002BOX EP09606 Life Tech. Life Tech. Life Tech. 40 10 5 10 each 10 each 8 each 1 100 g 1 1 each 50 g 1 each 15 96 each 15 96 each 45 24 each 25 8 each 15 25 each 105 (various) 5 100 ft 1 25 each HVLP14250 Sigma-Aldrich GE Healthcare Merck Millipore 5 50 each 162-0115 162-0117 162-0177 162-0174 IB301002 IB301001 170-4156 170-4157 170-3932 EI9052 Bio-Rad Bio-Rad Bio-Rad Bio-Rad Life Tech. Life Tech. Bio-Rad Bio-Rad Bio-Rad Life Tech. 20 10 30 5 45 100 20 180 85 35 1 each 10 each 1 each 10 each 10 each 10 each 10 each 10 each 50 each 8 each EC6865BOX G521802 G700802 Life Tech. Life Tech. Life Tech. 15 10 15 10 each 18 each 8 each 5.B Gel filtration, ion exchange Gel filtration reagents Sephadex G-50 Superfine 17-0041-01 Ion exchange resins Gel filtration LMW calibration kit Chelex 100 resin 28-4038-41 142-1253 Pre-packed columns for ion exchange HiScreen Capto DEAE 28-9269-82 Amicon 0.5 ml (3K) UFC500396 Amicon 4 ml (30K) UFC803096 Amicon 15 ml (10K) UFC901024 Centricon 70 ml (3K) UFC703008 GE Healthcare GE Healthcare Bio-Rad GE Healthcare Filtration/concentration, desalting, 5.C dialysis Protein sample concentrators Merck Millipore Merck Millipore Merck Millipore Merck Millipore Reverse phase columns Desalting columns Zeba Desalting spin columns (0.5 ml) Dialysis tubing and cassettes Slide-A-Lyzer dialysis cassettes Dialysis tubing, cellulose membrane (43 mm) Filter membranes Protran BA85, 137 mm Durapore membrane filters 89882 66380, etc. D9527 10401147 Thermo Fisher Thermo Fisher Blotting membraner, transfer stacks 5.D and paper Nitrocellulose PVDF Disposable transfer stacks Blotting paper and sponges NC membrane (roll, 33 cm x 3 m) NC membrane (pre-cut, 9 x 12 cm) PVDF membrane (roll, 26 cm x 3.3 m) PVDF membrane (pre-cut, 7 x 8.4 cm) iBlot stack, NC (mini) iBlot stack, NC (regular) Turbo stack, PVDF (mini) Turbo stack, PVDF (regular) Blotting filter papers Sponge pad for blotting Pre-cast gels for agarose gel 5.E electrophoresis 6% TBE-Urea gels, 10-well (1.0 mm) E-Gel 2%, with SYBR Safe, 12-well E-Gel 2%, 96-well Accessories for 5.F bacterial/yeast/plant/insect cell culture Sealing membranes Breathe-Easy sealing membrane Z380059-1PAK Sigma-Aldrich 1 100 each Bottle filter liners Dri-gauze filter liner Z710881-5EA Sigma-Aldrich 1 5 each 170-3931 170-4089 Bio-Rad Bio-Rad 5 1 1 each 1 each 5.G PAGE and Western blot accessories Gel holder Assay tray Product Basket 6 EQUIPMENT Pos Product bags no Article name ref ref cat no supplier volume pack each /year Size 6.A PAGE and Western blot equipment Protein gel electrophoresis cells XCell EI0001 Life Tech. 23 1 each WR0100 Life Tech. 5 1 each Gel blotting apparatuses XCell Midi Trans-Blot Turbo Blotting System 170-4155 Bio-Rad 9 1 each Power supplies for electrophoresis and blotting PowerPac Universal Power Supply 164-5070 Bio-Rad 1 1 each Mini-Sub Cell GT system 170-4406 Bio-Rad 1 1 each Sub Cell GT system 170-4401 Bio-Rad 1 1 each 1 each 6.B Equipment for nucleic acid determination Product Basket 7 MOLECULAR BIOLOGY KITS Pos Product bags no 7.A In vitro transcription and translation kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.B Transfection/transformation kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.C RNA kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.D Cloning kits INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.E RT-PCR and cDNA synthesis kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.F PCR kits and arrays INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.G Gene expression kits/reagents Gene expression kits Gene expression master mixes Gene expression endogenous probes Array cards 7.H Mutagenesis kits Directed mutagenesis kits Random mutagenesis kits 7.I Genotyping assay kits (incl. Chip kits) INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.J Library preparation kits INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.K Sequencing kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.L Western blot CL detection and protein quantification kits Western blotting substrate kits Protein quantification kits 7.M DNA purification and quantification kits Miniprep DNA purification kits Midiprep DNA purification kits Maxiprep, megaprep, gigaprep DNA purification kits Genomic DNA purification kits and reagents Gel extraction kits PCR purification and nucleotide removal kits DNA concentration kits Sequencing reaction cleanup kits Plant DNA purification kits DNA quantification kits Kits/chips for DNA Fragment analysis 7.N Magnetic bead isolation kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.O Epigenetics kits INGEN ARTIKEL ANGIVEN - ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.P Assay kits/reagents Metabolic and reporter assay kits Metabolic and reporter assay reagents 7.Q Assay kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.R Next-generation sequencing kits/reagents INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.S Protein purification kits INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT 7.T Other kits/reagents Kits and reagents for purification of biomolecules from cells and tissues Kits for protein crystallization Stock solutions for protein crystallization Protein isolation and purification kits