Product Basket 1 ENZYMES 1.A Restriction endonucleases

ENZYMES
Product Basket 1
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no
Product bags
Article name
pack
pack
size, amount
size, units
25
20
30
30
20
25
10
15
15
15
15
35
15
15
15
5
15
25
5
15
35
40
10000
2000
1000
10000
4000
10000
4000
1000
1000
4000
1000
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2000
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5000
Units
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Units
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Units
Units
Units
Units
10966034 Life Tech.
D8045 Sigma-Aldrich
11304011 Life Tech.
10342020 Life Tech.
100
35
25
100
300
500
100
500
rxn.
U
rxn.
Units
18010017
M0210S
M0203S
M0274S
1,088E+10
P2083
P1085
5
20
15
5
5
5
10
250
200
150
300
1 000
1 000
1 000
Units
Units
Units
Units
Units
Units
Units
30
30
160
40
25
25
30
90
30
550
50
15
2 500
100
10 000
2 500
1 000
10 000
3 000
20 000
2 000
1 500
70
100
Units
Units
Units
Units
Units
Units
Units
Units
Units
Units
Kunitz units
mg
reference
reference
cat no
supplier
R0136S
R0144S
R0176S
R0101S
R0195S
R0104S
R3142S
R0198S
R0193S
R0111S
R0131S
R0189S
R0156S
R0157S
R0138S
R3122S
R0141S
R0133S
R0182S
R3132L
R0145S
R0146S
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
NEB
volumes
1.A Restriction endonucleases
Restriction endonucleases
BamH I
Bgl II
Dpn I
EcoR I
EcoR V
Hind III
Kpn I (high fidelity)
Mlu I
Nco I
Nde I
Nhe I
Not I
Sac I
Sac II
Sal I
Sca I (HF)
Sma I
Spe I
Sph I
Ssp I (high fidelity)
Xba I
Xho I
1.B DNA polymerases for PCR
Hot start polymerases for PCR Platinum Taq DNA polymerase
High fidelity polymerases for P AccuTaq LA DNA polymerase
Hot start high fidelity polymeraPlatinum Taq DNA polymerase High Fidelity
Other polymerases for PCR Taq DNA polymerase
1.C DNA/RNA polymerases
DNA polymerases
RNA polymerases
DNA polymerase I
DNA polymerase I, large (Klenow) fragment
T4 DNA polymerase
T7 DNA polymerase
T7 RNA polymerase
T3 RNA polymerase
SP6 RNA polymerase
Life Tech.
NEB
NEB
NEB
Roche
Promega
Promega
1.D Enzymes for protein/DNA purification/modification
Transferases
Terminal transferase
Topoisomerases/recombinase Topoisomerase I (E. coli)
Reverse transcriptases
Superscript II reverse transcriptase
NA kinases
Polynucleotide kinase (E. coli)
NA phosphatases
Calf intestinal phosphatase (CIP)
Endonucleases
S1 nuclease
Exonucleases
Exo I nuclease
Ligases
T4 DNA ligase
T4 RNA ligase
DNAses
DNAse (RNAse-free) (50 rxn.)
RNAses
RNAse A from bovine pancreas
Proteinase
Proteinase K (5 ml)
Other enzymes for protein/DNA purification/modification
M0315S
NEB
M0301S
NEB
18064014 Life Tech.
M0201L
NEB
M0290S
NEB
M5761
Promega
M0293S
NEB
M0202S
NEB
M0351S
NEB
79254
Qiagen
R6513-10MG
Sigma-Aldric
25530049 Life Tech.
1.E Enzymes for Glycobiology
Carbohydrate-active enzymeEndoglycosidase H (500 ul)
Lysozyme from chicken egg white
PNGase F
11643053001
Roche
1
3
L6876
Sigma-Aldrich
35
5
Units
g
P0705S
NEB
25
15 000
Units
1.F Master mixes for PCR and qPCR
SYBR Green master mixes
Power SYBR Green PCR master mix (5 ml)
Master mixes without SYBR Gr TaqMan Fast Universal PCR master mix (2X)
4367659
4352042
Life Tech.
Life Tech.
120
135
reference
reference
volumes
cat no
supplier
100 bp DNA ladder
1 kb DNA ladder
RNA markers
NativeMark Unstained Protein Standard
SeeBlue Plus2 (stained protein standard)
15628019
10787018
G3191
LC0725
LC5925
Life Tech.
Life Tech.
Promega
Life Tech.
Life Tech.
dNTP set (individual dNTPs) (4 x 25 umol)
dNTP mix, 10 mM each (0.5 ml)
rNTP set (100 mM each (400 ul))
10297018 Life Tech.
D7295 Sigma-Aldrich
E6000
Promega
200
250
rxn.
rxn.
Product Basket 2 BIOPOLYMERS
Pos
Product bags
no
Article name
pack
pack
size, amount
size, units
30
25
10
10
100
50
250
50
250
500
ug
ug
ug
ul
ul
40
30
5
100
5
10
umol
umol
umol
25
25
10
25
5
nmol
nmol
nmol
ug
nmol
1500
150
50
50
50
25
10
40
nmol
ug
nmol
nmol
2.A Molecular weight standards
DNA MW standards
RNA MW standards
Protein MW standards
2.B dNTP, NTP, nucleosides
dNTPs
NTPs
2.C Primers (standard)
PCR primers and cloning oligosPrimer (tube) 20 b, 25 nmol, desalted
10629186 Life Tech. 100000
Primer (96- or 384-well plate) Primer (96- or 384-well plate), 20 primers, eac 10629152 Life Tech. 10000
Long oligonucleotides (30-120 Unmodified DNA oligo, 35 b, 10 nmol, desalteN001-001 (15ordika/Eurog 3500
T7 promoter primer, 2 ug, desalted
18418012 Life Tech.
Specific sequence primers
500
1000
Random primers
Primer (random hexamer), 5 nmol
N8080127 Life Tech.
2.D Primers (custom), RNA oligonucleotides, synthetic genes
Modified primers
qPCR probes
Primer 20 b (5'-Alexa-488), 50 nmol, HPLC
10623064 Life Tech.
Primer (oligo(dT)12-18, 5'-phosphorylated) 25 18418012 Life Tech.
qPCR probes (fluorescent end-labeled), 10 nm 450007
Life Tech.
qPCR probes (double-labeled: 5'-FAM + 3'-BHQB-DD601-004
ordika/Eurog
RNA oligonucleotides
Synthetic genes (>120 b)
2.E DNA (genomic); RNA (total, siRNA, other)
eukaryotic DNA
viral DNA
total RNA
siRNA
Other RNA
Salmon sperm DNA
15632011
Life Tech.
40
5x1
ml
FlexiTube siRNA
1027415
Qiagen
90
4x1
nmol
30
1
10
198
ug
clones
2.F Clones and gene libraries (cDNA, PAC, BAC)
cDNA clones
Human cDNA ORF clones
RC215923 Nordika/Orig
Human Entry Collection GPCR (glycerol stock) OHS4838 Thermo Fishe
2.G Vectors
Cloning vectors
pBR322
Expression vectors
pCDNA3.1
Reporter expression vectors
Vectors for cell-free expression
Reporter expression vectors
Vectors for cell-free expression
D1511
V79020
Promega
Life Tech.
5
1
10
20
ug
ug
P-MANIV
O-CPE
Megazyme
Megazyme
1
1
3
30
g
mg
1
10
1
100
g
ug
2.H Polysaccharides
Structural polysaccharides
Oligosaccharides
Mannan
Cellopentaose
2.I Peptides and recombinant proteins
Peptides
Recombinant proteins
di-peptide (e.g. Ala-Ala or equivalent)
A9502-1G Sigma-Aldrich
Recombinant protein (E.g. Histone H10 or equ M2501S
NEB
4. MOLECULAR BIOLOGY CHEMICALS
pack
pack
size, amount
size, units
25
25
25
500
500
400
ml
ml
ml
Sigma-Aldrich
Life Tech.
Life Tech.
Bio-Rad
Life Tech.
Life Tech.
80
100
100
45
125
50
25
500
500
5
10
250
ml
ml
ml
L
ml
ul
R90115
Life Tech.
20
25
ml
P3296
Sigma-Aldrich
5
1
ml
28324-P
Thermo
Fisher
50
60
ml
Tween-20
P5927
Sigma-Aldrich
15
100
ml
Triton X-100
T8787
Sigma-Aldrich
30
100
ml
16500500
S33102
Life Tech.
Life Tech.
40
125
500
400
g
ul
10777019
15581044
19086
402824
12041677001
10977035
239901
Life Tech.
Life Tech.
Qiagen
Life Tech.
Roche
Life Tech.
Qiagen
180
100
100
25
10
300
25
5 000
1
250
25
100
500
3
U
L
ml
ml
ml
ml
ml
170-6435
Bio-Rad
10
1
L
Pos
ref
ref
cat no
supplier
40% Acrylamide/Bisacrylamide Solution
2% Bisacrylamide Solution
UltraPure 10% SDS solution
161-0148
161-0142
15553027
Bio-Rad
Bio-Rad
Life Tech.
TEMED
MOPS (20X)
MES (20X)
TRIS-Glycine-SDS (10X)
LDS sample buffer (4X)
Reducing agent (10X)
T9281
NP0001
NP0002
161-0772
NP0007
NP0004
Product bags (Mol. Biol. Grade only!)
volumes
Article name
no
4.A PAGE reagents
Acrylamide, bisacrylamide
Solutions
Running buffers
Sample buffers and reagents
Transfer buffers
4.B Affinity purification
Affinity resins for IMAC
Protein A/G resins
Other affinity resins
Pre-packed columns for affinity purification
Ni-NTA agarose
Protein G sepharose fast flow
4.C Detergents for protein purification
NP-40 (10%)
Chemicals for NA synthesis,
purification, modification and
4.D determination
Agarose
NA stain
UltraPure agarose
SYBR Safe (10,000X)
RNAse inhibitors and surface decontaminants
Buffers for NA analysis
RNAseOUT RNAse inhibitor (recombinant)
TBE (10X)
EB
Running buffer (10X)
CDP-Star, ready-to-use
Water, DNAse/RNAse free
GelPilot DNA Loading dye (5X)
NA blot detection reagents
Purified/treated water
Loading dyes
Chemicals for protein synthesis,
purification, modification and
4.E determination
Blocking reagents and buffers for WB
TRIS buffer saline (TBS) 10X
Protease inhibitors
BSA Fraction V
cOmplete protease inhibitor cocktail tablets
A7906-100G
4693116001
Sigma-Aldrich
Roche
5
35
100
20
g
each
Protein inhibitors
Expression inducers/screening reagents
PhosSTOP phosphatase inhibitor cocktail tablets
X-Gal
4906837001
V3941
5
10
20
100
each
mg
5
25
g
5
50
mg
Cross-linking reagents
EZ-link NHS-LC-Biotin
21336
Roche
Promega
Saveen
Werner
Thermo
Fisher
Reducing agents
DTT, aqueous solution, 1M (10 x 0.5 ml)
646563
Sigma-Aldrich
15
5
mmol
ProLong Gold (or equivalent)
Dextran, tetramethylrhodamine
AccuCheck Counting Beads
Hoechst 33342
P36931
D1818
PCB100
H3570
Life Tech.
Life Tech.
Life Tech.
Life Tech.
60
20
20
10
10
25
10
10
ml
mg
ml
ml
IPTG
I1000-25
Chemicals for flow cytometry and
4.F labeling products
Antifade reagents
Dextrans
Fluorescent beads
FC cell labels
Product Basket 5 ACCESSORIES / DISPOSABLES
Pos
no
Product bags
Article name
ref
ref
cat no
supplier
NP0301BOX
Life Tech.
volume
pack
each /year Size
5.A Pre-cast PAGE gels
Constant percentage gels
NuPAGE gels, 10-well (10%, 1.0 mm)
75
10 each
NuPAGE gels, 10-well (10%, 1.5 mm)
NuPAGE gels, 12-well (12%, 1.0 mm)
NuPAGE gels, Tris-Acetate, 12-well (7%, 1.0
mm)
Gradient gels
Native gels
High-throughtput gels
NuPage gels, 10-well (4-12%, 1.0 mm)
NuPage gels, 10-well (4-12%, 1.5 mm)
NuPage gels, 12-well (4-12%, 1.0 mm)
NuPage gels, 15-well (4-12%, 1.0 mm)
NuPage gels, 17-well (4-12%, 1.0 mm)
Novex Midi gels, 26-well (4-12%)
NuPage Tris-Acetate gels, 12-well (3-8%, 1.0
mm)
NativePAGE gels, 10-well (4-16%, 1.0 mm)
E-PAGE 6 gels (6%)
NP0315BOX
NP0342BOX
Life Tech.
Life Tech.
100
65
10 each
10 each
EA03552BOX
NP0321BOX
NP0335BOX
NP0322BOX
NP0323BOX
NP0329BOX
WG1403BOX
Life Tech.
Life Tech.
Life Tech.
Life Tech.
Life Tech.
Life Tech.
Life Tech.
10
370
290
305
150
45
60
10 each
10 each
10 each
10 each
10 each
10 each
10 each
EA03752BOX
BN1002BOX
EP09606
Life Tech.
Life Tech.
Life Tech.
40
10
5
10 each
10 each
8 each
1
100 g
1
1
each
50 g
1
each
15
96 each
15
96 each
45
24 each
25
8 each
15
25 each
105
(various)
5
100 ft
1
25 each
HVLP14250
Sigma-Aldrich
GE
Healthcare
Merck
Millipore
5
50 each
162-0115
162-0117
162-0177
162-0174
IB301002
IB301001
170-4156
170-4157
170-3932
EI9052
Bio-Rad
Bio-Rad
Bio-Rad
Bio-Rad
Life Tech.
Life Tech.
Bio-Rad
Bio-Rad
Bio-Rad
Life Tech.
20
10
30
5
45
100
20
180
85
35
1 each
10 each
1 each
10 each
10 each
10 each
10 each
10 each
50 each
8 each
EC6865BOX
G521802
G700802
Life Tech.
Life Tech.
Life Tech.
15
10
15
10 each
18 each
8 each
5.B Gel filtration, ion exchange
Gel filtration reagents
Sephadex G-50 Superfine
17-0041-01
Ion exchange resins
Gel filtration LMW calibration kit
Chelex 100 resin
28-4038-41
142-1253
Pre-packed columns for ion exchange
HiScreen Capto DEAE
28-9269-82
Amicon 0.5 ml (3K)
UFC500396
Amicon 4 ml (30K)
UFC803096
Amicon 15 ml (10K)
UFC901024
Centricon 70 ml (3K)
UFC703008
GE
Healthcare
GE
Healthcare
Bio-Rad
GE
Healthcare
Filtration/concentration, desalting,
5.C dialysis
Protein sample concentrators
Merck
Millipore
Merck
Millipore
Merck
Millipore
Merck
Millipore
Reverse phase columns
Desalting columns
Zeba Desalting spin columns (0.5 ml)
Dialysis tubing and cassettes
Slide-A-Lyzer dialysis cassettes
Dialysis tubing, cellulose membrane (43 mm)
Filter membranes
Protran BA85, 137 mm
Durapore membrane filters
89882
66380, etc.
D9527
10401147
Thermo
Fisher
Thermo
Fisher
Blotting membraner, transfer stacks
5.D and paper
Nitrocellulose
PVDF
Disposable transfer stacks
Blotting paper and sponges
NC membrane (roll, 33 cm x 3 m)
NC membrane (pre-cut, 9 x 12 cm)
PVDF membrane (roll, 26 cm x 3.3 m)
PVDF membrane (pre-cut, 7 x 8.4 cm)
iBlot stack, NC (mini)
iBlot stack, NC (regular)
Turbo stack, PVDF (mini)
Turbo stack, PVDF (regular)
Blotting filter papers
Sponge pad for blotting
Pre-cast gels for agarose gel
5.E electrophoresis
6% TBE-Urea gels, 10-well (1.0 mm)
E-Gel 2%, with SYBR Safe, 12-well
E-Gel 2%, 96-well
Accessories for
5.F bacterial/yeast/plant/insect cell culture
Sealing membranes
Breathe-Easy sealing membrane
Z380059-1PAK
Sigma-Aldrich
1
100 each
Bottle filter liners
Dri-gauze filter liner
Z710881-5EA
Sigma-Aldrich
1
5 each
170-3931
170-4089
Bio-Rad
Bio-Rad
5
1
1 each
1 each
5.G PAGE and Western blot accessories
Gel holder
Assay tray
Product Basket 6 EQUIPMENT
Pos
Product bags
no
Article name
ref
ref
cat no
supplier
volume
pack
each /year Size
6.A PAGE and Western blot equipment
Protein gel electrophoresis cells
XCell
EI0001
Life Tech.
23
1 each
WR0100
Life Tech.
5
1 each
Gel blotting apparatuses
XCell Midi
Trans-Blot Turbo Blotting
System
170-4155
Bio-Rad
9
1 each
Power supplies for electrophoresis and blotting
PowerPac Universal
Power Supply
164-5070
Bio-Rad
1
1 each
Mini-Sub Cell GT system
170-4406
Bio-Rad
1
1 each
Sub Cell GT system
170-4401
Bio-Rad
1
1 each
1 each
6.B Equipment for nucleic acid determination
Product Basket 7 MOLECULAR BIOLOGY KITS
Pos
Product bags
no
7.A
In vitro transcription and translation kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.B Transfection/transformation kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.C RNA kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.D Cloning kits
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.E RT-PCR and cDNA synthesis kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.F PCR kits and arrays
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.G Gene expression kits/reagents
Gene expression kits
Gene expression master mixes
Gene expression endogenous probes
Array cards
7.H Mutagenesis kits
Directed mutagenesis kits
Random mutagenesis kits
7.I Genotyping assay kits (incl. Chip kits)
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.J Library preparation kits
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.K Sequencing kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.L Western blot CL detection and protein quantification kits
Western blotting substrate kits
Protein quantification kits
7.M DNA purification and quantification kits
Miniprep DNA purification kits
Midiprep DNA purification kits
Maxiprep, megaprep, gigaprep DNA purification kits
Genomic DNA purification kits and reagents
Gel extraction kits
PCR purification and nucleotide removal kits
DNA concentration kits
Sequencing reaction cleanup kits
Plant DNA purification kits
DNA quantification kits
Kits/chips for DNA Fragment analysis
7.N Magnetic bead isolation kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.O Epigenetics kits
INGEN ARTIKEL ANGIVEN - ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.P Assay kits/reagents
Metabolic and reporter assay kits
Metabolic and reporter assay reagents
7.Q Assay kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.R Next-generation sequencing kits/reagents
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.S Protein purification kits
INGA REFERENSARTIKLAR- ANBUSGIVARE ANGAV ARTIKLAR UR EGET SORTIMENT
7.T Other kits/reagents
Kits and reagents for purification of biomolecules from cells and tissues
Kits for protein crystallization
Stock solutions for protein crystallization
Protein isolation and purification kits