Department of Molecular Biology
Course 5MO018
Linda Sandblad
Schema VT 2013
Genetik och Geneteknik
Genetics and Genetic Techniques
Page 1 (5)
Course information: 5MO018, Genetic and genetic
techniques, 7.5 hp.
Course dates:
January 21 – February 20, 2013
Exam:
February 20. Time: 9:00-13:00
Localities:
Lecture room: “Gamla Biblioteket” building 6K middle floor
(Plan 0)
Laboratory: “Blå Lab” building 6L
Course Literature: 1) Genetics - From Genes to Genomes, 4th edition (2011). Hartwell
L.H., Hood L., Goldberg M.L., Reynolds A.E., Silver L.M. Mc-GrawHill.
2) Supplementary information given out during lectures and
laboratory exercises and problem solving classes.
Absences:
Absence from mandatory exercises due to illness should
immediately be reported to the course secretary
Contact information:
Course leader:
Linda Sandblad, tel: 090 785 0818,
[email protected]
Assistants:
Hau Xu, [email protected]
Ummehan Savas, [email protected]
Course Secretary: Eva-Christine Lundström, tel 090 785 2869,
[email protected]
Lecturers: Anders Byström (AB), Bernt Eric Uhlin (BEU), Christer Larsson (CL), Erik
Lundgren (EL), Edouard Pesquet (EP), Jan Larsson (JL), Linda Sandblad (LS), Matthew
Francis (MF), Monica Holmberg (MH), Nelson Gekara (NG), Victoria Shingler (VS),
Yuri Schwartz (YS)
Department of Molecular Biology
Course 5MO018
Linda Sandblad
Schema VT 2013
Genetik och Geneteknik
Genetics and Genetic Techniques
Page 2 (5)
Overview of lectures, labs and problem solving classes:
5MO018, Genetic and genetic techniques, 7.5 hp.
Lectures:
1.
The central dogma revisited (LS)
2.
The key tools of the geneticist - mutations, selections and screens (VS)
3.
Gene transfer, DNA cloning and genetic analysis in bacteria I (LS)
4.
Gene transfer, DNA cloning and genetic analysis in bacteria II (CL)
5.
Molecular genetic techniques: Detecting DNA, RNA and proteins (LS)
6.
Molecular genetic techniques: PCR (MF)
7.
Molecular genetic techniques: DNA sequencing and DNA-protein interaction
techniques (LS)
8.
Molecular genetic techniques: Homologous recombination and allelic
replacement in bacteria (CL)
9.
Gene and operon fusion (VS)
10.
Mendelian genetics and the principles of inheritance (JL)
11.
Linkage, recombination and gene mapping (JL)
12.
Yeast genetics I (AB)
13.
Yeast genetics II (AB)
14.
Yeast genetics III (AB)
15.
DNA repair - infidelity mistakes as a source for mutations (BEU)
16.
Locating disease associated alleles (MH)
17.
Genetic model organism – Mouse (NG)
18.
Genetic model organism – Drosophila (YS)
19.
Gene ethics (EL)
20.
Plant biotechnology and transgenic plants (EP)
21.
Genetic model organisms – Arabidopsis (EP)
Labs:
Lab 1:
Lab 2:
Lab 3:
Lab 4:
Lab 5:
Transfer of genetic material between bacteria via conjugation
PCR – introducing a desired mutation
Allelic replacement of a gene in E. coli
Yeast cell types and mating
Yeast complementation analysis
Problem solving classes:
PSC 1: Codons, reading frames and mutations
PSC 2: Restriction enzymes, cloning and complementation
PSC 3: Northern, Sothern, Western
PSC 4: Gene fusions and reporters
PSC 5: Genetic problems – Yeast genetics
Department of Molecular Biology
Course 5MO018
Linda Sandblad
Schema VT 2013
Genetik och Geneteknik
Genetics and Genetic Techniques
Page 3 (5)
Kurs: Genetik och genteknik, 7,5 hp 5MO018 – VT 2013
mån 2013-01-21
09:00 - 09:45
Role call and introduction with overview of the course Var:
Gamla Biblioteket
10:00 - 12:00
Lecture (LS): The central dogma revisited Var: Gamla
Biblioteket
13:30 - 15:30
Lecture (VS): The key tools of the geneticist - mutations,
selections and screens Var: Gamla Biblioteket
tis 2013-01-22
10:00 - 12:00
13:00 - 15:00
15:00 - 17:00
Lecture (LS): Gene transfer, DNA cloning and genetic analysis
in bacteria I Var: Gamla Biblioteket
Lecture (CL): Gene transfer, DNA cloning and genetic analysis
in bacteria II Var: Gamla Biblioteket
Problem solving class 1 (LS) Var: Gamla Biblioteket
ons 2013-01-23
09:00 - 11:00
Lecture (LS): Molecular genetic techniques: Detecting DNA,
RNA and proteins Var: Gamla Biblioteket
11:00 - 12:30
Lab safari and safety. Introduction to Lab 1 – Transfer of
genetic material between bacteria via conjugation (Hau Xu)
Var: Gamla Biblioteket
13:30 - 15:00
Lab 1 Day 1 Var: Blå lab
15:00 - 17:00
Lecture (MF): Molecular genetic techniques: PCR Var: Gamla
Biblioteket
tor 2013-01-24
09:00 - 11:00
14:00 - 17:00
Lecture (LS): Molecular genetic techniques: DNA sequencing
and DNA-protein interaction techniques Var: Gamla
Biblioteket
Lecture (CL): Molecular genetic techniques: Homologous
recombination and allelic replacement in bacteria Var: Gamla
Biblioteket
Lab 1 Day 2 Var: Blå lab
fre 2013-01-25
09:00 - 11:00
11:00 - 12:00
13:00 - 15:00
Lecture (VS): Gene and operon fusion Var: Gamla Biblioteket
Problem solving class 2 (LS) Var: Gamla Biblioteket
Lab 1 Day 3 Var: Blå lab
12:00 - 14:00
mån 2013-01-28
09:00 - 10:00
Introduction to Lab 2 – PCR – introducing a desired mutation
(Ummehan Savas) Var: Gamla Biblioteket
10:00 - 17:00
Lab 2 Day 1 Var: Blå lab
Department of Molecular Biology
Course 5MO018
Linda Sandblad
Schema VT 2013
Genetik och Geneteknik
Genetics and Genetic Techniques
Page 4 (5)
tis 2013-01-29
Uniaden, undervisningsfri dag
ons 2013-01-30
09:00 - 11:00
Lecture (JL): Mendelian genetics and the principles of
inheritance Var: Gamla Biblioteket
11:00 - 12:00
Problem solving class 3 (LS) Var: Gamla Biblioteket
13:00 - 17:00
Lab 2 Day 2 Var: Blå lab
tor 2013-01-31
09:00 - 11:00
11:00 - 12:00
13:00 - 17:00
Lecture (JL): Linkage, recombination and gene mapping Var:
Gamla Biblioteket
Problem solving class 4 (LS) Var: Gamla Biblioteket
Lab 2 Day 3 Var: Blå lab
fre 2013-02-01
09:00 - 11:00
12:00 - 14:00
14:00 - 17:00
Lecture (AB): Yeast genetics I Var: Gamla Biblioteket
Lecture (AB): Yeast Genetics II Var: Gamla Biblioteket
Lab 2 Day 4 Var: Blå lab
mån 2013-02-04
09:00 - 11:00
Lecture (AB): Yeast genetics III Var: Gamla Bibilioteket
11:00 - 12:00
Introduction to Lab 3 – Allelic replacement of a gene in E. coli
(Ummehan Savas) Var: Gamla Biblioteket
13:00 - 17:00
Lab 2 Day 5, Lab 3 Day 1 Var: Blå lab
tis 2013-02-05
09:00 - 17:00
Lab 2 Day 6, Lab 3 Day 2 Var: Blå lab
ons 2013-02-06
09:00 - 11:00
Lecture (BEU): DNA repair - infidelity mistakes as a source for
mutations Var: Gamla Biblioteket
11:00 - 12:00
Introduction to Lab 4 and 5 - Yeast cell types, mating and
complementation analysis (Hau Xu) Var: Gamla Bibilioteket
13:00 - 17:00
Lab 3 Day 3, Lab 5 Day 1 Var: Blå lab
tor 2013-02-07
09:00 - 11:00
11:00 - 12:00
13:00 - 17:00
13:00 - 17:00
Lecture (MH): Locating disease associated alleles Var: Gamla
Biblioteket
Problem solving class 5 Var: Gamla Biblioteket
Lab 2 day 7 Var: Gamla Biblioteket
Lab 3 Day 4, Lab 5 Day 2 Var: Blå lab
Department of Molecular Biology
Course 5MO018
Linda Sandblad
fre 2013-02-08
09:00 - 17:00
09:00 - 17:00
Schema VT 2013
Genetik och Geneteknik
Genetics and Genetic Techniques
Page 5 (5)
Lab 2 Day 8 Var: Gamla Biblioteket
Lab 3 Day 5, Lab 5 Day 3 Var: Blå lab
mån 2013-02-11
09:00 - 11:00
Lecture (NG): Genetic model organism – Mouse Var: Gamla
Biblioteket
12:00 - 17:00
Lab 3 Day 6, Lab 4 Day 1, Lab 5 Day 4 Var: Blå lab
tis 2013-02-12
09:00 - 11:00
11:00 - 12:00
12:00 - 17:00
Lecture (YS): Genetic model organism – Drosophila Var:
Gamla Biblioteket
Lab 4 Day 2 Var: Blå lab
Fettisdagen, undervisningsfri em
ons 2013-02-13
09:00 - 11:00
Lecture (EL): Gene ethics Var: Gamla Biblioteket
12:00 - 17:00
Lab 3 Day 7, Lab 4 Day 3, Lab 5 Day 5 Var: Blå lab
tor 2013-02-14
09:00 - 11:00
12:00 - 17:00
fre 2013-02-15
09:00 - 11:00
12:00 - 15:00
Lecture (EP): Plant biotechnology and transgenic plants Var:
Gamla Biblioteket
Lab 3 Day 8, Lab 5 Day 6 and clean up Var: Blå lab
Lecture (EP): Genetic model organisms – Arabidopsis Var:
Gamla Biblioteket
Lab round-up and question time (Assistants) Var: Gamla
Biblioteket
mån 2013-02-18
09:00 - 11:00
Question time (LS) Var: Gamla Biblioteket
tis 2013-02-19
Private study
ons 2013-02-20
09:00 - 13:00
Exam, room 6, Östra Paviljongen
